Proteomic Analysis

Peptides and proteins identified by re-processing the raw mass-spectrometry data of every proteomic dataset with a single documented pipeline (Comet 2026.01 against the CnidoSite reference proteome plus cRAP contaminants, with Percolator target-decoy FDR control). Search parameters are dataset-specific and are shown in full for each dataset; peptides were mapped onto CnidoSite gene models, so every row links to its gene page.

Dataset
Contaminants

Dataset provenance & search parameters — PXD060643

SpeciesActinia fragacea
Tissuenematocysts, various tissues
PRIDE accessionPXD060643
Statusreprocessed
Search engineComet 2026.01
Reference proteome/mnt/sdb/jackie/cnidaria_omics/transcriptome_proteins/Actinia_tenebrosa.rep.pep
Enzymetrypsin
Missed cleavages2
Precursor tolerance10 ppm
Fragment tolerance0.02 Da
Fixed modificationC+57.021464
Variable modificationM+15.9949
Decoy strategyComet internal reversed (1:1)
FDR (PSM)0.01
Quantificationspectral counting (PSMs)
Proteins4572
Peptides (q≤0.01)24717
PSMs (q≤0.01)130483
reprocessed against the CnidoSite reference proteome
15 cRAP contaminant protein s (keratins, trypsin, serum albumin …) were identified in this dataset and are excluded from the table below. Tick “include cRAP contaminants” to display them.
Gene Protein PSMs Unique peptides Coverage (%) Length Best q-value Description
TRINITY_DN698_c0_g1 TRINITY_DN698_c0_g1_i1.p1 1 1 5 242 0.00811309
TRINITY_DN6990_c0_g1 TRINITY_DN6990_c0_g1_i1.p1 1 1 2.1 526 0.00672478
TRINITY_DN70_c7_g2 TRINITY_DN70_c7_g2_i1.p1 1 1 1.2 1468 0.0000713063
TRINITY_DN701_c0_g1 TRINITY_DN701_c0_g1_i1.p1 1 1 1 1039 0.000262312
TRINITY_DN701_c6_g1 TRINITY_DN701_c6_g1_i1.p1 1 1 3.9 203 0.00131081
TRINITY_DN7020_c0_g2 TRINITY_DN7020_c0_g2_i1.p1 1 1 0.9 997 0.00563167
TRINITY_DN7040_c1_g2 TRINITY_DN7040_c1_g2_i1.p1 1 1 2.5 550 0.0000713063
TRINITY_DN708_c1_g1 TRINITY_DN708_c1_g1_i3.p1 1 1 0.6 1801 0.000646064
TRINITY_DN711_c0_g1 TRINITY_DN711_c0_g1_i1.p1 1 1 5.3 283 0.0000713063
TRINITY_DN7120_c0_g1 TRINITY_DN7120_c0_g1_i1.p1 1 1 1.4 875 0.000819169
TRINITY_DN72_c17_g1 TRINITY_DN72_c17_g1_i1.p1 1 1 1.6 817 0.000646064
TRINITY_DN7279_c0_g1 TRINITY_DN7279_c0_g1_i1.p1 1 1 3 403 0.000556886
TRINITY_DN729_c1_g2 TRINITY_DN729_c1_g2_i1.p1 1 1 5.7 175 0.00168555
TRINITY_DN7302_c1_g1 TRINITY_DN7302_c1_g1_i2.p1 1 1 0.9 770 0.00876799
TRINITY_DN7321_c0_g2 TRINITY_DN7321_c0_g2_i1.p1 1 1 3.2 505 0.0000713063
TRINITY_DN7324_c0_g1 TRINITY_DN7324_c0_g1_i1.p2 1 1 1.5 686 0.0000713063
TRINITY_DN7328_c0_g1 TRINITY_DN7328_c0_g1_i4.p1 1 1 5.3 302 0.0000713063
TRINITY_DN7341_c1_g3 TRINITY_DN7341_c1_g3_i2.p1 1 1 2.8 425 0.000646064
TRINITY_DN7357_c0_g1 TRINITY_DN7357_c0_g1_i2.p1 1 1 3 264 0.00147178
TRINITY_DN7384_c0_g1 TRINITY_DN7384_c0_g1_i1.p1 1 1 5.5 165 0.000596432
TRINITY_DN75_c0_g1 TRINITY_DN75_c0_g1_i12.p1 1 1 6.2 146 0.0000713063
TRINITY_DN753_c0_g2 TRINITY_DN753_c0_g2_i1.p1 1 1 3.1 287 0.000359389
TRINITY_DN7553_c7_g1 TRINITY_DN7553_c7_g1_i1.p1 1 1 2.1 518 0.00176802
TRINITY_DN7564_c0_g1 TRINITY_DN7564_c0_g1_i4.p1 1 1 5.4 223 0.0052656
TRINITY_DN7589_c0_g1 TRINITY_DN7589_c0_g1_i3.p1 1 1 5 220 0.0000713063
Total protein records: 4572  (+15 contaminants hidden)
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