Proteomic Analysis

Peptides and proteins identified by re-processing the raw mass-spectrometry data of every proteomic dataset with a single documented pipeline (Comet 2026.01 against the CnidoSite reference proteome plus cRAP contaminants, with Percolator target-decoy FDR control). Search parameters are dataset-specific and are shown in full for each dataset; peptides were mapped onto CnidoSite gene models, so every row links to its gene page.

Dataset
Contaminants

Dataset provenance & search parameters — PXD060643

SpeciesActinia fragacea
Tissuenematocysts, various tissues
PRIDE accessionPXD060643
Statusreprocessed
Search engineComet 2026.01
Reference proteome/mnt/sdb/jackie/cnidaria_omics/transcriptome_proteins/Actinia_tenebrosa.rep.pep
Enzymetrypsin
Missed cleavages2
Precursor tolerance10 ppm
Fragment tolerance0.02 Da
Fixed modificationC+57.021464
Variable modificationM+15.9949
Decoy strategyComet internal reversed (1:1)
FDR (PSM)0.01
Quantificationspectral counting (PSMs)
Proteins4572
Peptides (q≤0.01)24717
PSMs (q≤0.01)130483
reprocessed against the CnidoSite reference proteome
15 cRAP contaminant protein s (keratins, trypsin, serum albumin …) were identified in this dataset and are excluded from the table below. Tick “include cRAP contaminants” to display them.
Gene Protein PSMs Unique peptides Coverage (%) Length Best q-value Description
TRINITY_DN7604_c0_g1 TRINITY_DN7604_c0_g1_i1.p1 1 1 1.7 814 0.00895765
TRINITY_DN7623_c0_g1 TRINITY_DN7623_c0_g1_i14.p1 1 1 0.7 1409 0.00438484
TRINITY_DN763_c0_g1 TRINITY_DN763_c0_g1_i1.p1 1 1 12.6 119 0.0000713063
TRINITY_DN7661_c0_g3 TRINITY_DN7661_c0_g3_i1.p1 1 1 4.1 410 0.00247436
TRINITY_DN7661_c0_g7 TRINITY_DN7661_c0_g7_i2.p1 1 1 1.3 834 0.0000713063
TRINITY_DN7690_c0_g1 TRINITY_DN7690_c0_g1_i2.p1 1 1 3.8 290 0.00567298
TRINITY_DN77_c4_g1 TRINITY_DN77_c4_g1_i1.p1 1 1 3.2 343 0.00351761
TRINITY_DN770_c0_g1 TRINITY_DN770_c0_g1_i1.p1 1 1 1.4 1033 0.000596432
TRINITY_DN7716_c0_g1 TRINITY_DN7716_c0_g1_i1.p1 1 1 12.9 101 0.00164551
TRINITY_DN7836_c0_g1 TRINITY_DN7836_c0_g1_i1.p1 1 1 1.6 625 0.000359389
TRINITY_DN784_c1_g2 TRINITY_DN784_c1_g2_i1.p1 1 1 1.8 443 0.00113412
TRINITY_DN7871_c0_g1 TRINITY_DN7871_c0_g1_i1.p1 1 1 1.9 619 0.0000713063
TRINITY_DN7872_c0_g2 TRINITY_DN7872_c0_g2_i6.p1 1 1 7.4 229 0.0000713063
TRINITY_DN7901_c0_g1 TRINITY_DN7901_c0_g1_i1.p1 1 1 11.3 151 0.0000713063
TRINITY_DN7902_c0_g1 TRINITY_DN7902_c0_g1_i3.p1 1 1 3 365 0.00030849
TRINITY_DN7976_c0_g1 TRINITY_DN7976_c0_g1_i2.p1 1 1 8.2 110 0.00426858
TRINITY_DN7985_c0_g1 TRINITY_DN7985_c0_g1_i4.p1 1 1 7.4 135 0.000413126
TRINITY_DN8037_c0_g1 TRINITY_DN8037_c0_g1_i1.p1 1 1 1.9 750 0.000141804
TRINITY_DN8112_c1_g1 TRINITY_DN8112_c1_g1_i2.p1 1 1 2.2 632 0.00343687
TRINITY_DN8128_c0_g1 TRINITY_DN8128_c0_g1_i1.p1 1 1 3.4 435 0.0000713063
TRINITY_DN8150_c1_g1 TRINITY_DN8150_c1_g1_i11.p1 1 1 4.4 295 0.0000713063
TRINITY_DN8177_c0_g1 TRINITY_DN8177_c0_g1_i1.p1 1 1 3 234 0.00458462
TRINITY_DN818_c0_g2 TRINITY_DN818_c0_g2_i3.p1 1 1 8.5 130 0.00388299
TRINITY_DN821_c6_g1 TRINITY_DN821_c6_g1_i6.p1 1 1 9 279 0.0000713063
TRINITY_DN821_c7_g3 TRINITY_DN821_c7_g3_i2.p1 1 1 1.6 685 0.000456413
Total protein records: 4572  (+15 contaminants hidden)
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