Proteomic Analysis

Peptides and proteins identified by re-processing the raw mass-spectrometry data of every proteomic dataset with a single documented pipeline (Comet 2026.01 against the CnidoSite reference proteome plus cRAP contaminants, with Percolator target-decoy FDR control). Search parameters are dataset-specific and are shown in full for each dataset; peptides were mapped onto CnidoSite gene models, so every row links to its gene page.

Dataset
Contaminants

Dataset provenance & search parameters — PXD060643

SpeciesActinia fragacea
Tissuenematocysts, various tissues
PRIDE accessionPXD060643
Statusreprocessed
Search engineComet 2026.01
Reference proteome/mnt/sdb/jackie/cnidaria_omics/transcriptome_proteins/Actinia_tenebrosa.rep.pep
Enzymetrypsin
Missed cleavages2
Precursor tolerance10 ppm
Fragment tolerance0.02 Da
Fixed modificationC+57.021464
Variable modificationM+15.9949
Decoy strategyComet internal reversed (1:1)
FDR (PSM)0.01
Quantificationspectral counting (PSMs)
Proteins4572
Peptides (q≤0.01)24717
PSMs (q≤0.01)130483
reprocessed against the CnidoSite reference proteome
15 cRAP contaminant protein s (keratins, trypsin, serum albumin …) were identified in this dataset and are excluded from the table below. Tick “include cRAP contaminants” to display them.
Gene Protein PSMs Unique peptides Coverage (%) Length Best q-value Description
TRINITY_DN8953_c0_g1 TRINITY_DN8953_c0_g1_i2.p1 1 1 2.2 858 0.0000713063
TRINITY_DN90_c0_g1 TRINITY_DN90_c0_g1_i2.p1 1 1 0.7 1989 0.000262312
TRINITY_DN91_c0_g1 TRINITY_DN91_c0_g1_i11.p1 1 1 7.6 185 0.0000713063
TRINITY_DN911_c1_g4 TRINITY_DN911_c1_g4_i1.p1 1 1 7.3 178 0.00176802
TRINITY_DN9164_c3_g1 TRINITY_DN9164_c3_g1_i1.p1 1 1 7.4 175 0.00147178
TRINITY_DN9182_c0_g1 TRINITY_DN9182_c0_g1_i2.p1 1 1 1.1 1210 0.000202375
TRINITY_DN9228_c0_g1 TRINITY_DN9228_c0_g1_i8.p1 1 1 8 410 0.00101302
TRINITY_DN9328_c0_g1 TRINITY_DN9328_c0_g1_i3.p1 1 1 2 564 0.00168555
TRINITY_DN9513_c0_g1 TRINITY_DN9513_c0_g1_i1.p1 1 1 4 350 0.0000713063
TRINITY_DN9540_c0_g1 TRINITY_DN9540_c0_g1_i1.p1 1 1 2.6 302 0.00694473
TRINITY_DN960_c0_g3 TRINITY_DN960_c0_g3_i2.p1 1 1 5.6 216 0.000506443
TRINITY_DN960_c0_g6 TRINITY_DN960_c0_g6_i1.p1 1 1 5.5 470 0.0000713063
TRINITY_DN9638_c0_g1 TRINITY_DN9638_c0_g1_i1.p1 1 1 2.9 444 0.000202375
TRINITY_DN964_c0_g1 TRINITY_DN964_c0_g1_i6.p1 1 1 4.2 192 0.00194022
TRINITY_DN9660_c0_g1 TRINITY_DN9660_c0_g1_i1.p1 1 1 11.3 266 0.00426858
TRINITY_DN9667_c0_g1 TRINITY_DN9667_c0_g1_i1.p1 1 1 1.5 844 0.000202375
TRINITY_DN9748_c0_g1 TRINITY_DN9748_c0_g1_i2.p1 1 1 3.6 281 0.00311882
TRINITY_DN9882_c0_g1 TRINITY_DN9882_c0_g1_i6.p1 1 1 4.1 219 0.000596432
TRINITY_DN9884_c0_g1 TRINITY_DN9884_c0_g1_i1.p2 1 1 3.9 311 0.00155521
TRINITY_DN990_c0_g4 TRINITY_DN990_c0_g4_i1.p1 1 1 4.2 356 0.0000713063
TRINITY_DN9974_c0_g1 TRINITY_DN9974_c0_g1_i2.p1 1 1 9.9 111 0.0000713063
TRINITY_DN998_c7_g1 TRINITY_DN998_c7_g1_i10.p1 1 1 4.2 236 0.0000713063
Total protein records: 4572  (+15 contaminants hidden)
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