Proteomic Analysis

Peptides and proteins identified by re-processing the raw mass-spectrometry data of every proteomic dataset with a single documented pipeline (Comet 2026.01 against the CnidoSite reference proteome plus cRAP contaminants, with Percolator target-decoy FDR control). Search parameters are dataset-specific and are shown in full for each dataset; peptides were mapped onto CnidoSite gene models, so every row links to its gene page.

Dataset
Contaminants

Dataset provenance & search parameters — PXD060643

SpeciesActinia fragacea
Tissuenematocysts, various tissues
PRIDE accessionPXD060643
Statusreprocessed
Search engineComet 2026.01
Reference proteome/mnt/sdb/jackie/cnidaria_omics/transcriptome_proteins/Actinia_tenebrosa.rep.pep
Enzymetrypsin
Missed cleavages2
Precursor tolerance10 ppm
Fragment tolerance0.02 Da
Fixed modificationC+57.021464
Variable modificationM+15.9949
Decoy strategyComet internal reversed (1:1)
FDR (PSM)0.01
Quantificationspectral counting (PSMs)
Proteins4572
Peptides (q≤0.01)24717
PSMs (q≤0.01)130483
reprocessed against the CnidoSite reference proteome
15 cRAP contaminant protein s (keratins, trypsin, serum albumin …) were identified in this dataset and are excluded from the table below. Tick “include cRAP contaminants” to display them.
Gene Protein PSMs Unique peptides Coverage (%) Length Best q-value Description
TRINITY_DN47900_c0_g1 TRINITY_DN47900_c0_g1_i1.p1 420 24 42.1 387 0.0000713063
TRINITY_DN9771_c0_g1 TRINITY_DN9771_c0_g1_i2.p1 383 24 43.7 517 0.0000713063
TRINITY_DN385_c6_g1 TRINITY_DN385_c6_g1_i1.p1 339 24 61.5 514 0.0000713063
TRINITY_DN39088_c0_g1 TRINITY_DN39088_c0_g1_i1.p1 254 24 47.5 568 0.0000713063
TRINITY_DN600_c0_g1 TRINITY_DN600_c0_g1_i1.p1 203 24 59.7 427 0.0000713063
TRINITY_DN18192_c0_g1 TRINITY_DN18192_c0_g1_i1.p1 201 24 65.3 418 0.0000713063
TRINITY_DN3342_c7_g1 TRINITY_DN3342_c7_g1_i1.p1 197 24 40.1 434 0.0000713063
TRINITY_DN30_c0_g1 TRINITY_DN30_c0_g1_i31.p1 192 24 38.6 531 0.0000713063
TRINITY_DN636_c1_g1 TRINITY_DN636_c1_g1_i26.p1 179 24 37.5 554 0.0000713063
TRINITY_DN10602_c0_g1 TRINITY_DN10602_c0_g1_i2.p1 134 24 54.5 402 0.0000713063
TRINITY_DN9149_c0_g2 TRINITY_DN9149_c0_g2_i1.p1 116 24 37.1 652 0.0000713063
TRINITY_DN24887_c0_g1 TRINITY_DN24887_c0_g1_i1.p1 93 24 15.4 1153 0.0000713063
TRINITY_DN398_c0_g1 TRINITY_DN398_c0_g1_i12.p1 77 24 20.1 1224 0.0000713063
TRINITY_DN54507_c0_g1 TRINITY_DN54507_c0_g1_i1.p1 70 24 33 959 0.0000713063
TRINITY_DN8331_c0_g1 TRINITY_DN8331_c0_g1_i2.p1 66 24 21.5 1408 0.0000713063
TRINITY_DN3497_c0_g1 TRINITY_DN3497_c0_g1_i2.p1 356 23 60.7 252 0.0000713063
TRINITY_DN840_c0_g1 TRINITY_DN840_c0_g1_i1.p1 267 23 70.9 148 0.0000713063
TRINITY_DN15573_c0_g1 TRINITY_DN15573_c0_g1_i3.p1 224 23 41.9 547 0.0000713063
TRINITY_DN27453_c0_g1 TRINITY_DN27453_c0_g1_i1.p1 216 23 62.9 426 0.0000713063
TRINITY_DN42540_c0_g1 TRINITY_DN42540_c0_g1_i1.p1 177 23 67.7 269 0.0000713063
TRINITY_DN7484_c0_g1 TRINITY_DN7484_c0_g1_i6.p1 167 23 57 442 0.0000713063
TRINITY_DN7894_c0_g1 TRINITY_DN7894_c0_g1_i3.p1 149 23 36.3 531 0.0000713063
TRINITY_DN3738_c1_g2 TRINITY_DN3738_c1_g2_i2.p1 137 23 47.4 428 0.0000713063
TRINITY_DN391_c3_g3 TRINITY_DN391_c3_g3_i1.p1 122 23 50.5 566 0.0000713063
TRINITY_DN7233_c0_g2 TRINITY_DN7233_c0_g2_i1.p1 101 23 55.3 490 0.0000713063
Total protein records: 4572  (+15 contaminants hidden)
Show: proteins per page
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