« All proteomic datasets | Browse the 559 identified proteins »
| Parameter | As reported by the original study | As applied by CnidoSite |
|---|---|---|
| Search engine | SEQUEST / MScDb | Comet 2026.01 + Percolator (Crux 4.2) |
| Sequence database | custom Aiptasia DB (320,798 seq) | – CnidoSite reference proteome (2.anno), BUSCO-validated |
| Enzyme | – (not restated) | trypsin (fully enzymatic, 2 termini) · up to 2 missed cleavages |
| Precursor tolerance | not stated | 10 ppm |
| Fragment tolerance | not stated | 0.5 Da |
| Fixed modification | – | C+57.021464 (carbamidomethyl cysteine) |
| Variable modification | – | M+15.9949 (methionine oxidation) |
| Decoy strategy | – | Comet internal reversed (1:1) |
| False discovery rate | peptide/protein validation | q ≤ 0.01 at the PSM level (Percolator q-values, target-decoy); protein level q ≤ 0.01 |
| Quantification | – | spectral counting (PSMs) |
| Contaminants | – | cRAP appended and flagged; 5 contaminant proteins identified and excluded from the reported protein list |
| Gene mapping | – | Peptides mapped to CnidoSite gene models; each protein links to its gene page |
| PSMs at q≤0.01 | Peptides at q≤0.01 | Gene-linked proteins | Contaminants (excluded) |
|---|---|---|---|
| 2,523 | 1,673 | 559 | 5 |
Pipeline version cnidosite-proteomics 1.0 · CnidoSite release 1.1 · incorporated 2026-09-18
Every stage is a single command; the parameter file written by stage 2 is the one shown in the table above.
| Gene | Protein | Unique peptides | PSMs | Coverage (%) | Length | Best q | Description |
|---|---|---|---|---|---|---|---|
| KXJ17389.1 | EDIAP_KXJ17389.1 | 39 | 61 | 12.8 | 2825 | 0.000882613 | hypothetical protein AC249_AIPGENE23779 [Exaiptasia diaphana] |
| KXJ26779.1 | EDIAP_KXJ26779.1 | 34 | 51 | 58.5 | 520 | 0.000882613 | hypothetical protein AC249_AIPGENE26430 [Exaiptasia diaphana] |
| KXJ10693.1 | EDIAP_KXJ10693.1 | 28 | 44 | 51 | 341 | 0.000882613 | Protein mab-21-like 2 [Exaiptasia diaphana] |
| KXJ28063.1 | EDIAP_KXJ28063.1 | 27 | 39 | 66.1 | 363 | 0.000882613 | Mitogen-activated protein kinase kinase kinase kinase 5 [Exaiptasia diaphana] |
| KXJ11682.1 | EDIAP_KXJ11682.1 | 26 | 48 | 29.5 | 376 | 0.000882613 | Beta-glucuronidase [Exaiptasia diaphana] |
| KXJ09142.1 | EDIAP_KXJ09142.1 | 26 | 47 | 60.3 | 478 | 0.000882613 | hypothetical protein AC249_AIPGENE15663 [Exaiptasia diaphana] |
| KXJ18066.1 | EDIAP_KXJ18066.1 | 21 | 30 | 45.6 | 509 | 0.000882613 | hypothetical protein AC249_AIPGENE21454 [Exaiptasia diaphana] |
| KXJ23430.1 | EDIAP_KXJ23430.1 | 19 | 39 | 82.9 | 164 | 0.000882613 | Tetratricopeptide repeat protein 38 [Exaiptasia diaphana] |
| KXJ25073.1 | EDIAP_KXJ25073.1 | 17 | 30 | 44.1 | 392 | 0.000882613 | Trace amine-associated receptor 7d [Exaiptasia diaphana] |
| KXJ16139.1 | EDIAP_KXJ16139.1 | 17 | 21 | 29.1 | 554 | 0.000882613 | Cytosolic carboxypeptidase 1 [Exaiptasia diaphana] |
| KXJ26570.1 | EDIAP_KXJ26570.1 | 16 | 32 | 7.5 | 1944 | 0.000882613 | [Protein ADP-ribosylarginine] hydrolase [Exaiptasia diaphana] |
| KXJ15233.1 | EDIAP_KXJ15233.1 | 16 | 29 | 27.6 | 424 | 0.000882613 | hypothetical protein AC249_AIPGENE838 [Exaiptasia diaphana] |
| KXJ15109.1 | EDIAP_KXJ15109.1 | 16 | 23 | 12.9 | 1708 | 0.000882613 | Major facilitator superfamily domain-containing protein 6 [Exaiptasia diaphana] |
| KXJ17104.1 | EDIAP_KXJ17104.1 | 15 | 33 | 79.3 | 150 | 0.000882613 | Proteasome assembly chaperone 1 [Exaiptasia diaphana] |
| KXJ20935.1 | EDIAP_KXJ20935.1 | 14 | 25 | 26.1 | 713 | 0.000882613 | Coagulation factor VIII [Exaiptasia diaphana] |
