« All proteomic datasets | Browse the 3451 identified proteins »
| Parameter | As reported by the original study | As applied by CnidoSite |
|---|---|---|
| Search engine | MaxQuant (Andromeda) | Comet 2026.01 + Percolator (Crux 4.2) |
| Sequence database | Trinity+TransDecoder assembly of the same animal (Amir-min_assembly_longest_250_transdecoder.fasta) | – CnidoSite reference proteome (2.anno), BUSCO-validated |
| Enzyme | – (not restated) | trypsin/P (fully enzymatic, 2 termini) · up to 2 missed cleavages |
| Precursor tolerance | 20 ppm first / 4.5 ppm main | 10 ppm |
| Fragment tolerance | not stated | 0.5 Da |
| Fixed modification | – | C+57.021464 (carbamidomethyl cysteine) |
| Variable modification | – | M+15.9949 (methionine oxidation) |
| Decoy strategy | – | Comet internal reversed (1:1) |
| False discovery rate | 1% | q ≤ 0.01 at the PSM level (Percolator q-values, target-decoy); protein level q ≤ 0.01 |
| Quantification | – | spectral counting (PSMs) |
| Contaminants | – | cRAP appended and flagged; 20 contaminant proteins identified and excluded from the reported protein list |
| Gene mapping | – | Peptides mapped to CnidoSite gene models; each protein links to its gene page |
| PSMs at q≤0.01 | Peptides at q≤0.01 | Gene-linked proteins | Contaminants (excluded) |
|---|---|---|---|
| 377,023 | 14,806 | 3,451 | 20 |
Pipeline version cnidosite-proteomics 1.0 · CnidoSite release 1.1 · incorporated 2026-09-20
Every stage is a single command; the parameter file written by stage 2 is the one shown in the table above.
| Gene | Protein | Unique peptides | PSMs | Coverage (%) | Length | Best q | Description |
|---|---|---|---|---|---|---|---|
| KXJ17389.1 | EDIAP_KXJ17389.1 | 190 | 8861 | 54.7 | 2825 | 0.000121477 | hypothetical protein AC249_AIPGENE23779 [Exaiptasia diaphana] |
| KXJ13133.1 | EDIAP_KXJ13133.1 | 190 | 6169 | 24.5 | 9496 | 0.000121477 | Agrin [Exaiptasia diaphana] |
| KXJ26570.1 | EDIAP_KXJ26570.1 | 176 | 15011 | 55.2 | 1944 | 0.000121477 | [Protein ADP-ribosylarginine] hydrolase [Exaiptasia diaphana] |
| KXJ26578.1 | EDIAP_KXJ26578.1 | 123 | 4387 | 52.3 | 1966 | 0.000121477 | hypothetical protein AC249_AIPGENE8218 [Exaiptasia diaphana] |
| KXJ13091.1 | EDIAP_KXJ13091.1 | 109 | 2583 | 40.3 | 2871 | 0.000121477 | Peroxisomal biogenesis factor 19 [Exaiptasia diaphana] |
| KXJ09111.1 | EDIAP_KXJ09111.1 | 104 | 1595 | 14 | 10714 | 0.000121477 | DNA-binding protein SATB1 [Exaiptasia diaphana] |
| KXJ11690.1 | EDIAP_KXJ11690.1 | 94 | 1589 | 11.2 | 10785 | 0.000121477 | High affinity cationic amino acid transporter 1 [Exaiptasia diaphana] |
| KXJ15312.1 | EDIAP_KXJ15312.1 | 93 | 3365 | 46.6 | 2353 | 0.000121477 | Sushi, von Willebrand factor type A, EGF and pentraxin domain-containing protein 1 [Exaiptasia diaphana] |
| KXJ25225.1 | EDIAP_KXJ25225.1 | 90 | 2014 | 36.7 | 2799 | 0.000121477 | hypothetical protein AC249_AIPGENE22509 [Exaiptasia diaphana] |
| KXJ20344.1 | EDIAP_KXJ20344.1 | 89 | 3092 | 58.6 | 1682 | 0.000121477 | Alpha-tubulin N-acetyltransferase [Exaiptasia diaphana] |
| KXJ15583.1 | EDIAP_KXJ15583.1 | 85 | 5371 | 44 | 2001 | 0.000121477 | hypothetical protein AC249_AIPGENE10299 [Exaiptasia diaphana] |
| KXJ28015.1 | EDIAP_KXJ28015.1 | 84 | 1061 | 8.8 | 13504 | 0.000121477 | Phosphatidylinositol N-acetylglucosaminyltransferase subunit H [Exaiptasia diaphana] |
| KXJ26681.1 | EDIAP_KXJ26681.1 | 76 | 1545 | 37.7 | 2065 | 0.000121477 | putative rRNA-processing protein EBP2 [Exaiptasia diaphana] |
| KXJ17380.1 | EDIAP_KXJ17380.1 | 73 | 1498 | 23.5 | 3661 | 0.000121477 | DNA helicase MCM9 [Exaiptasia diaphana] |
| KXJ30222.1 | EDIAP_KXJ30222.1 | 72 | 2598 | 28.8 | 3185 | 0.000121477 | Mannose-1-phosphate guanyltransferase beta-B [Exaiptasia diaphana] |
