Proteomic Dataset Details

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PXD051329 — Exaiptasia diaphana — Cyst structure

SpeciesExaiptasia diaphana
ClassHexacorallia
Tissue / sampleCyst structure
Treatment / conditionHost-Parasite Infection
InstrumentQ Exactive HF-X
PRIDE project PXD051329
Publication PMID 38891869
Raw files10

Original study vs CnidoSite re-processing

Parameter As reported by the original study As applied by CnidoSite
Search engine not stated in the deposit Comet 2026.01 + Percolator (Crux 4.2)
Sequence database not stated in the deposit
CnidoSite reference proteome (2.anno), BUSCO-validated
Enzyme (not restated) trypsin (fully enzymatic, 2 termini)  ·  up to 2 missed cleavages
Precursor tolerance not stated 10 ppm
Fragment tolerance not stated 0.02 Da
Fixed modification C+57.021464 (carbamidomethyl cysteine)
Variable modification M+15.9949 (methionine oxidation)
Decoy strategy Comet internal reversed (1:1)
False discovery rate not stated q ≤ 0.01 at the PSM level (Percolator q-values, target-decoy); protein level q ≤ 0.01
Quantification spectral counting (PSMs)
Contaminants cRAP appended and flagged; 20 contaminant proteins identified and excluded from the reported protein list
Gene mapping Peptides mapped to CnidoSite gene models; each protein links to its gene page

Outcome of re-processing

Re-processed successfully. reprocessed against the CnidoSite reference proteome
PSMs at q≤0.01Peptides at q≤0.01 Gene-linked proteinsContaminants (excluded)
14,764 878 298 20

Pipeline version cnidosite-proteomics 1.0 · CnidoSite release 1.1 · incorporated 2026-09-20

Reproducing this dataset

Every stage is a single command; the parameter file written by stage 2 is the one shown in the table above.

python3 2.pipeline/02_fetch_pride.py PXD051329 # retrieve peak lists from PRIDE python3 2.pipeline/03_build_search_db.py "Exaiptasia diaphana" # proteome + cRAP python3 2.pipeline/04_run_search.py PXD051329 # writes comet.params, runs Comet python3 2.pipeline/05_fdr_percolator.py PXD051329 # Percolator, q ≤ 0.01 python3 2.pipeline/06_map_to_genes.py PXD051329 # peptides -> CnidoSite genes python3 2.pipeline/07_build_tables.py --release 1.1 # load tables + SQL

Most strongly supported proteins in this dataset

GeneProtein Unique peptidesPSMsCoverage (%) LengthBest qDescription
KXJ11485.1 EDIAP_KXJ11485.1 31 1076 52.8 447 0.00148368 Phosphatidate cytidylyltransferase, mitochondrial [Exaiptasia diaphana]
KXJ11682.1 EDIAP_KXJ11682.1 29 1304 41.5 376 0.00148368 Beta-glucuronidase [Exaiptasia diaphana]
KXJ10478.1 EDIAP_KXJ10478.1 23 1113 50.7 134 0.00148368 hypothetical protein AC249_AIPGENE9907 [Exaiptasia diaphana]
KXJ20864.1 EDIAP_KXJ20864.1 18 148 19.4 803 0.00148368 Lipid phosphate phosphohydrolase 3 [Exaiptasia diaphana]
KXJ16139.1 EDIAP_KXJ16139.1 12 266 19.7 554 0.00148368 Cytosolic carboxypeptidase 1 [Exaiptasia diaphana]
KXJ21663.1 EDIAP_KXJ21663.1 11 586 25.3 376 0.00148368 Protein arginine N-methyltransferase 3 [Exaiptasia diaphana]
KXJ16990.1 EDIAP_KXJ16990.1 11 341 25.9 556 0.00148368 Transmembrane matrix receptor MUP-4 [Exaiptasia diaphana]
KXJ10758.1 EDIAP_KXJ10758.1 10 216 22.6 288 0.00148368 Coiled-coil domain-containing protein 34 [Exaiptasia diaphana]
KXJ08383.1 EDIAP_KXJ08383.1 9 309 38.5 340 0.00148368 Uncharacterized protein K02A2.6 [Exaiptasia diaphana]
KXJ20389.1 EDIAP_KXJ20389.1 9 144 10.3 857 0.00148368 Sodium-coupled monocarboxylate transporter 1 [Exaiptasia diaphana]
KXJ10548.1 EDIAP_KXJ10548.1 9 80 47.2 218 0.00148368 Acylamino-acid-releasing enzyme [Exaiptasia diaphana]
KXJ04510.1 EDIAP_KXJ04510.1 8 349 32.8 131 0.00148368 hypothetical protein AC249_AIPGENE5933, partial [Exaiptasia diaphana]
KXJ08381.1 EDIAP_KXJ08381.1 8 156 31.6 206 0.00148368 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, mitochondrial [Exaiptasia diaphana]
KXJ29013.1 EDIAP_KXJ29013.1 8 72 4.9 1674 0.00148368 Serine/threonine-protein kinase SIK3 [Exaiptasia diaphana]
KXJ29853.1 EDIAP_KXJ29853.1 7 144 8.1 726 0.00148368 MAGUK p55 subfamily member 5 [Exaiptasia diaphana]

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