Proteomic Analysis

Peptides and proteins identified by re-processing the raw mass-spectrometry data of every proteomic dataset with a single documented pipeline (Comet 2026.01 against the CnidoSite reference proteome plus cRAP contaminants, with Percolator target-decoy FDR control). Search parameters are dataset-specific and are shown in full for each dataset; peptides were mapped onto CnidoSite gene models, so every row links to its gene page.

Dataset
Contaminants

Dataset provenance & search parameters — PXD033068

SpeciesNematostella vectensis
TissueEmbryos
PRIDE accessionPXD033068
Statusreprocessed
Search engineComet 2026.01
Reference proteomeCnidoSite reference proteome
Enzymetrypsin
Missed cleavages2
Precursor tolerance10 ppm
Fragment tolerance0.02 Da
Fixed modificationC+57.021464
Variable modificationM+15.9949
Decoy strategyComet internal reversed (1:1)
FDR (PSM)0.01
Quantificationspectral counting (PSMs)
Proteins3673
Peptides (q≤0.01)20212
PSMs (q≤0.01)93156
reprocessed against the CnidoSite reference proteome
17 cRAP contaminant protein s (keratins, trypsin, serum albumin …) were identified in this dataset and are excluded from the table below. Tick “include cRAP contaminants” to display them.
Gene Protein PSMs Unique peptides Coverage (%) Length Best q-value Description
XP_048586175.1 NVECT_XP_048586175.1 42 14 45.1 441 0.0000678288 RNA-binding protein 4.1 isoform X1 [Nematostella vectensis]
XP_048587919.1 NVECT_XP_048587919.1 38 14 45.5 409 0.0000678288 alkaline ceramidase 3 isoform X1 [Nematostella vectensis]
XP_001635037.1 NVECT_XP_001635037.1 37 14 19.7 833 0.0000678288 proteasome subunit beta type-3 [Nematostella vectensis]
XP_048586587.1 NVECT_XP_048586587.1 36 14 18.8 921 0.0000678288 metastasis-associated protein MTA3 isoform X2 [Nematostella vectensis]
XP_001633338.3 NVECT_XP_001633338.3 163 13 66.4 140 0.0000678288 optic atrophy 3 protein homolog [Nematostella vectensis]
XP_032232826.2 NVECT_XP_032232826.2 112 13 58.7 172 0.0000678288 uncharacterized protein LOC5508150 isoform X1 [Nematostella vectensis]
XP_048587922.1 NVECT_XP_048587922.1 104 13 71.4 140 0.0000678288 DNA polymerase epsilon catalytic subunit A isoform X1 [Nematostella vectensis]
XP_032229003.1 NVECT_XP_032229003.1 103 13 52 367 0.0000678288 fibroblast growth factor 1 [Nematostella vectensis]
XP_001641608.3 NVECT_XP_001641608.3 91 13 60.7 275 0.0000678288 TNF receptor-associated factor 5 [Nematostella vectensis]
XP_001629439.3 NVECT_XP_001629439.3 89 13 74.2 217 0.0000678288 phosphoenolpyruvate phosphomutase [Nematostella vectensis]
XP_032241950.1 NVECT_XP_032241950.1 89 13 50.9 287 0.0000678288 MAM and LDL-receptor class A domain-containing protein 1 [Nematostella vectensis]
XP_048580957.1 NVECT_XP_048580957.1 87 13 59.1 1080 0.0000678288 uncharacterized protein LOC116606501 isoform X2 [Nematostella vectensis]
XP_001631702.1 NVECT_XP_001631702.1 85 13 60.7 262 0.0000678288 gamma-secretase subunit Aph-1 [Nematostella vectensis]
XP_048581375.1 NVECT_XP_048581375.1 85 13 20.6 688 0.0000678288 uncharacterized protein LOC116620186 isoform X1 [Nematostella vectensis]
XP_048577986.1 NVECT_XP_048577986.1 84 13 55.1 336 0.0000678288 solute carrier organic anion transporter family member 4A1 isoform X1 [Nematostella vectensis]
XP_048584453.1 NVECT_XP_048584453.1 82 13 44 366 0.0000678288 gelsolin-like protein 1 [Nematostella vectensis]
XP_048586428.1 NVECT_XP_048586428.1 76 13 58.2 297 0.0000678288 carbonic anhydrase 2 isoform X1 [Nematostella vectensis]
XP_032239289.1 NVECT_XP_032239289.1 73 13 46.9 326 0.0000678288 low density lipoprotein receptor adapter protein 1-A isoform X2 [Nematostella vectensis]
XP_048580259.1 NVECT_XP_048580259.1 73 13 25.9 428 0.0000678288 growth hormone secretagogue receptor type 1 [Nematostella vectensis]
XP_048587227.1 NVECT_XP_048587227.1 72 13 25.2 640 0.0000678288 far upstream element-binding protein 3 isoform X2 [Nematostella vectensis]
XP_001631360.2 NVECT_XP_001631360.2 68 13 36.6 402 0.0000678288 ras-related protein Rab-10 isoform X1 [Nematostella vectensis]
XP_001632974.3 NVECT_XP_001632974.3 66 13 62.6 227 0.0000678288 UPF0686 protein C11orf1 homolog [Nematostella vectensis]
XP_048579966.1 NVECT_XP_048579966.1 66 13 34.1 419 0.0000678288 methionine--tRNA ligase, cytoplasmic isoform X2 [Nematostella vectensis]
XP_048588188.1 NVECT_XP_048588188.1 66 13 32 547 0.0000678288 propionyl-CoA carboxylase beta chain, mitochondrial [Nematostella vectensis]
XP_001633848.1 NVECT_XP_001633848.1 64 13 52.5 263 0.0000678288 tripeptidyl-peptidase 1 [Nematostella vectensis]
Total protein records: 3673  (+17 contaminants hidden)
Show: proteins per page
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