Detailed information of KXJ20444.1 in Exaiptasia diaphana

Genomic Location: LJWW01000112.1:316386...319575
NR annotation: XP_020902891.1, RNA demethylase ALKBH5 [Exaiptasia diaphana]
Species Exaiptasia diaphana · all data for this species · gene families


 Gene Structure
More details in JBrowse  Sequence
CDS
Protein
 UniProt (Swiss-Prot top hit)
UniProt accessionDescription
Q6GPB5RNA demethylase ALKBH5 OS=Xenopus laevis OX=8355 GN=alkbh5 PE=2 SV=1
E1BH29RNA demethylase ALKBH5 OS=Bos taurus OX=9913 GN=ALKBH5 PE=3 SV=1
Q6P6C2RNA demethylase ALKBH5 OS=Homo sapiens OX=9606 GN=ALKBH5 PE=1 SV=2
 Gene family
Family typeMembership / link
Orthogroup (gene family)OG0008303 (this species only) · gene tree & orthology

 Pfam domain
Pfam accessionPfam nameDescriptionTypeSource
PF13532
all species →		2OG-FeII_Oxy_22OG-Fe(II) oxygenase superfamilyDomainInterproscan

 InterPro
InterPro termTypeDescriptionSource
IPR005123
all species →		DomainOxoglutarate/iron-dependent dioxygenaseInterproscan
IPR027450
all species →		DomainAlpha-ketoglutarate-dependent dioxygenase AlkB-likeInterproscan
IPR037151
all species →		Homologous_superfamilyAlpha-ketoglutarate-dependent dioxygenase AlkB-like superfamilyInterproscan
IPR032860
all species →		FamilyRNA demethylase ALKBH5Interproscan

 PANTHER
PANTHER termDescriptionSource
PTHR32074
all species →		RNA DEMETHYLASE ALKBH5Interproscan

 Gene Ontology
GO termCategoryDescriptionSource
GO:0005634
all species →		Cellular ComponentnucleusInterproscan
GO:0006397
all species →		Biological ProcessmRNA processingInterproscan
GO:0006406
all species →		Biological ProcessmRNA export from nucleusInterproscan
GO:0035515
all species →		Molecular Functionoxidative RNA demethylase activityInterproscan
GO:0035553
all species →		Biological Processobsolete oxidative single-stranded RNA demethylationInterproscan

Search by domain instead of by gene. Any accession above (InterPro, Pfam, PANTHER, GO, KEGG) can be used as a query on the Functional Domain Search page, which searches all 148 annotated genomes at once.
 KEGG pathway
KOEnzymeEnzyme IDPathwayMap IDSource
K10767ALKBH5; mRNA N6-methyladenine demethylaseEC:1.14.11.53
Messenger RNA biogenesisko03019deepkoala

Searching by KO or pathway ID across all species is available on the KEGG Pathway page.

Expression pattern (RNA-seq)

Transcript abundance of KXJ20444.1 across 72 RNA-seq samples of Exaiptasia diaphana. Values are TPM (transcripts per million) from the StringTie quantification; one bar is one sample, grouped and coloured by condition, sorted by expression within each group.

72Samples
68TPM > 0
2Conditions
68.9Max TPM
42.8Mean TPM

By condition

ConditionSamplesTPM > 0 Mean TPMMax TPMMean, relative to max
whole animal · aposymbiotic 36 34 40.64 68.87
whole animal · symbiotic 36 34 45.04 58.70

Per sample · hover a bar for the full sample record

Show the sample table (72 samples)
SRA runConditionTissueDevelopmental stage TreatmentStudyTPM
SRR6202357 whole animal · aposymbiotic whole animal not recorded aposymbiotic SRP120942 68.87
SRR6202234 whole animal · aposymbiotic whole animal not recorded aposymbiotic SRP120942 51.43
SRR6202260 whole animal · aposymbiotic whole animal not recorded aposymbiotic SRP120942 48.91
SRR6202242 whole animal · aposymbiotic whole animal not recorded aposymbiotic SRP120942 48.30
SRR6202233 whole animal · aposymbiotic whole animal not recorded aposymbiotic SRP120942 47.49
SRR6202208 whole animal · aposymbiotic whole animal not recorded aposymbiotic SRP120942 46.89
SRR6202259 whole animal · aposymbiotic whole animal not recorded aposymbiotic SRP120942 45.93
SRR6202261 whole animal · aposymbiotic whole animal not recorded aposymbiotic SRP120942 45.62
SRR6202238 whole animal · aposymbiotic whole animal not recorded aposymbiotic SRP120942 45.61
SRR6202346 whole animal · aposymbiotic whole animal not recorded aposymbiotic SRP120942 45.32
SRR6202258 whole animal · aposymbiotic whole animal not recorded aposymbiotic SRP120942 44.81
SRR6202209 whole animal · aposymbiotic whole animal not recorded aposymbiotic SRP120942 44.77
SRR6202344 whole animal · aposymbiotic whole animal not recorded aposymbiotic SRP120942 44.75
SRR6202204 whole animal · aposymbiotic whole animal not recorded aposymbiotic SRP120942 44.66
SRR6202256 whole animal · aposymbiotic whole animal not recorded aposymbiotic SRP120942 44.13
SRR6202206 whole animal · aposymbiotic whole animal not recorded aposymbiotic SRP120942 43.34
SRR6202240 whole animal · aposymbiotic whole animal not recorded aposymbiotic SRP120942 42.98
SRR6202239 whole animal · aposymbiotic whole animal not recorded aposymbiotic SRP120942 42.94
SRR6202207 whole animal · aposymbiotic whole animal not recorded aposymbiotic SRP120942 42.57
SRR6202257 whole animal · aposymbiotic whole animal not recorded aposymbiotic SRP120942 42.17
SRR6202235 whole animal · aposymbiotic whole animal not recorded aposymbiotic SRP120942 41.87
SRR6202236 whole animal · aposymbiotic whole animal not recorded aposymbiotic SRP120942 41.81
SRR6202254 whole animal · aposymbiotic whole animal not recorded aposymbiotic SRP120942 40.50
SRR6202212 whole animal · aposymbiotic whole animal not recorded aposymbiotic SRP120942 40.30
SRR6202203 whole animal · aposymbiotic whole animal not recorded aposymbiotic SRP120942 39.85
SRR6202255 whole animal · aposymbiotic whole animal not recorded aposymbiotic SRP120942 39.78
SRR6202237 whole animal · aposymbiotic whole animal not recorded aposymbiotic SRP120942 39.43
SRR6202211 whole animal · aposymbiotic whole animal not recorded aposymbiotic SRP120942 38.00
SRR6202210 whole animal · aposymbiotic whole animal not recorded aposymbiotic SRP120942 37.73
SRR6202241 whole animal · aposymbiotic whole animal not recorded aposymbiotic SRP120942 37.56
SRR6202363 whole animal · aposymbiotic whole animal not recorded aposymbiotic SRP120942 36.69
SRR6202205 whole animal · aposymbiotic whole animal not recorded aposymbiotic SRP120942 36.16
SRR6202343 whole animal · aposymbiotic whole animal not recorded aposymbiotic SRP120942 32.22
SRR6202358 whole animal · aposymbiotic whole animal not recorded aposymbiotic SRP120942 29.59
SRR6202345 whole animal · aposymbiotic whole animal not recorded aposymbiotic SRP120942 0.00
SRR6202364 whole animal · aposymbiotic whole animal not recorded aposymbiotic SRP120942 0.00
SRR6202337 whole animal · symbiotic whole animal not recorded symbiotic SRP120942 58.70
SRR6202283 whole animal · symbiotic whole animal not recorded symbiotic SRP120942 56.02
SRR6202308 whole animal · symbiotic whole animal not recorded symbiotic SRP120942 55.99
SRR6202310 whole animal · symbiotic whole animal not recorded symbiotic SRP120942 55.52
SRR6202342 whole animal · symbiotic whole animal not recorded symbiotic SRP120942 55.27
SRR6202285 whole animal · symbiotic whole animal not recorded symbiotic SRP120942 52.20
SRR6202282 whole animal · symbiotic whole animal not recorded symbiotic SRP120942 52.16
SRR6202352 whole animal · symbiotic whole animal not recorded symbiotic SRP120942 52.11
SRR6202304 whole animal · symbiotic whole animal not recorded symbiotic SRP120942 51.51
SRR6202305 whole animal · symbiotic whole animal not recorded symbiotic SRP120942 50.86
SRR6202338 whole animal · symbiotic whole animal not recorded symbiotic SRP120942 50.28
SRR6202280 whole animal · symbiotic whole animal not recorded symbiotic SRP120942 50.22
SRR6202351 whole animal · symbiotic whole animal not recorded symbiotic SRP120942 50.16
SRR6202307 whole animal · symbiotic whole animal not recorded symbiotic SRP120942 50.01
SRR6202347 whole animal · symbiotic whole animal not recorded symbiotic SRP120942 49.30
SRR6202276 whole animal · symbiotic whole animal not recorded symbiotic SRP120942 48.67
SRR6202306 whole animal · symbiotic whole animal not recorded symbiotic SRP120942 47.94
SRR6202349 whole animal · symbiotic whole animal not recorded symbiotic SRP120942 47.87
SRR6202278 whole animal · symbiotic whole animal not recorded symbiotic SRP120942 47.59
SRR6202355 whole animal · symbiotic whole animal not recorded symbiotic SRP120942 47.59
SRR6202303 whole animal · symbiotic whole animal not recorded symbiotic SRP120942 47.41
SRR6202277 whole animal · symbiotic whole animal not recorded symbiotic SRP120942 46.44
SRR6202281 whole animal · symbiotic whole animal not recorded symbiotic SRP120942 45.81
SRR6202339 whole animal · symbiotic whole animal not recorded symbiotic SRP120942 45.80
SRR6202356 whole animal · symbiotic whole animal not recorded symbiotic SRP120942 45.13
SRR6202309 whole animal · symbiotic whole animal not recorded symbiotic SRP120942 44.46
SRR6202284 whole animal · symbiotic whole animal not recorded symbiotic SRP120942 43.46
SRR6202263 whole animal · symbiotic whole animal not recorded symbiotic SRP120942 42.75
SRR6202262 whole animal · symbiotic whole animal not recorded symbiotic SRP120942 42.53
SRR6202350 whole animal · symbiotic whole animal not recorded symbiotic SRP120942 41.91
SRR6202341 whole animal · symbiotic whole animal not recorded symbiotic SRP120942 41.68
SRR6202279 whole animal · symbiotic whole animal not recorded symbiotic SRP120942 41.22
SRR6202340 whole animal · symbiotic whole animal not recorded symbiotic SRP120942 40.05
SRR6202348 whole animal · symbiotic whole animal not recorded symbiotic SRP120942 22.76
SRR6202353 whole animal · symbiotic whole animal not recorded symbiotic SRP120942 0.00
SRR6202354 whole animal · symbiotic whole animal not recorded symbiotic SRP120942 0.00

Source: CnidoSite RNA-seq expression matrices (EDIAP_TPM, StringTie quantification over 72 runs), joined to SRA sample metadata. Samples whose tissue/treatment is not recorded in the source metadata are grouped by the descriptor carried in the expression matrix itself.

Proteomic evidence

Peptides from this gene's protein product were identified in 3 re-processed proteomic datasets at a false discovery rate of q ≤ 0.01 (Comet + Percolator).

3Unique peptides
16PSMs
2.6%Max coverage
0.0001Best q-value
3Datasets
Dataset Species Tissue / condition Peptides PSMs Coverage Best q
PXD055908 Exaiptasia diaphana Whole anemone
Heat stress (32℃) vs Control 		1 9
2.6%
0.0000707064 peptides
K.GIEYTESYSDISTNPK.D q=0.0000707064
PXD045585 Exaiptasia diaphana Symbiont Breviolum minutum
Symbiosis establishment 		1 4
2.6%
0.000121477 peptides
K.GIEYTESYSDISTNPK.D q=0.000121477
PXD045587 Exaiptasia diaphana Symbiont Durusdinium trenchii
Symbiosis establishment 		1 3
2.6%
0.000174307 peptides
K.GIEYTESYSDISTNPK.D q=0.000174307

Search parameters for this evidence: Comet 2026.01, precursor 10 ppm, fragment 0.5 Da, fixed C+57.021464, variable M+15.9949,n+42.010565, PSM-level q ≤ 0.01 against . Parameters differ between datasets; open a dataset for its full provenance.  ·  all proteins for Exaiptasia diaphana

Co-expression network

Genes whose expression across the transcriptome samples of Exaiptasia diaphana tracks this one. Counts are over the whole network; the network view itself draws at most 100 partners per query gene (CNIDO_NET_TOP_K), so a hub gene can show fewer edges than the number below.

DirectionPartnersMost correlated partnerPCC
Positively correlated19KXJ19411.10.804108790384116
Negatively correlated13KXJ19325.1-0.716210176641294

Open this gene in Network Analysis → The network opens with the gene already entered and both directions selected. There you can add up to 9 more genes, switch between the positive and negative network, and export the edge list.

Single-cell expression

Whether this gene can be visualised in the single-cell atlases of Exaiptasia diaphana, and in which cell types it is a marker. Expression is stored per cell, so the violin plot and the cell-type means are computed in the viewer — open a dataset to see them.

No single-cell dataset has been published for this species. The atlases that do exist are listed in the Cell Atlas.

Epigenetic marks

Chromatin and DNA-methylation data covering this gene. Peak calls are listed per sample with the genomic region the peak falls in; DNA methylation is listed as sample availability only, because those tables are queried by the DNA Methylation page itself.

Peak calls overlapping this gene

AssaySamplePeaksRegion
ChIP-seqH3K27ac_symbiont1Promoter (<=1kb) 1
H3K9ac_symbiont1Promoter (<=1kb) 1
ATAC-seqMild_Stress_Aposym1Promoter (<=1kb) 1

Browse the full epigenomic landscape of this species: ChIP-seq · ATAC-seq.

DNA methylation (bisulphite samples)

SampleMethylation profile
whole_animal_aposymbiotic_1open
whole_animal_aposymbiotic_2open
whole_animal_aposymbiotic_3open
whole_animal_aposymbiotic_4open
whole_animal_aposymbiotic_5open
whole_animal_aposymbiotic_6open
whole_animal_aposymbiotic_7open
whole_animal_aposymbiotic_8open
whole_animal_aposymbiotic_9open
whole_animal_symbiotic_1open
whole_animal_symbiotic_10open
whole_animal_symbiotic_11open
whole_animal_symbiotic_12open
whole_animal_symbiotic_13open
whole_animal_symbiotic_14open
whole_animal_symbiotic_2open
whole_animal_symbiotic_3open
whole_animal_symbiotic_4open
whole_animal_symbiotic_5open
whole_animal_symbiotic_6open
whole_animal_symbiotic_7open
whole_animal_symbiotic_8open
whole_animal_symbiotic_9open

The DNA Methylation page locates this gene by scanning the bisulphite table, which holds one row per cytosine. For the largest datasets that scan takes a while to return; it does not mean the link is broken.

What you can do with this gene

Every tool below opens with this gene already entered, so you land on the analysis rather than on an empty form. Links open in a new tab.

AnalysisWhat it doesStatus
Primer designDesign PCR / qPCR primers on this gene’s sequence with the published primer3 settings. Opens with the primers already computed.template foundopen →
BLASTSearch this gene’s sequence against the CnidoSite BLAST databases. The query is filled in and the species’ database is preselected — press Run to start.template foundopen →
Expression heatmapDraw this gene’s expression as a heatmap across all RNA-seq samples, and compare it with the genes you add next to it.readyopen →
Gene family / orthogroupLook this gene up in the single-copy orthogroups built from the high-quality cnidarian genomes, and see which other species carry an orthologue.open →
Gene set analysisStart a gene-set enrichment analysis with this gene as the seed list. Add more genes on that page before running it.open →
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